Purify Kits
After you use the ChiroSolve Screening kits, these kits can be used to:
- Recover the racemate used during screening
- Use the recovered racemate to perform further re-crystallization steps to enrich the target enantiomer until highest or optimum purity is achieved
The kits are self-contained and do not require any other device (except HPLC to identify how much enrichment is achieved after each re-crystallization) to do the work. They are available in two sizes:
- Smaller size to be used along with a single screening kit
- Larger size to be used with a pack of four (4) screening kits
The kits include following main components:
- Recovery Solution that must be added to the content of screening kits after the screening experiment; so that the racemate can be liberated from the diastereomeric salts. The resultant mixture forms two layers:
- Top layer contains the resolving agents, water and solvents
- Bottom layer contains the racemate along with some solvents - Filtering unit that includes
- Filter funnel with vacuum adapter; so that enantiomeric crystals can be separated from the filtrate by using light vacuum during progressive re-crystallization steps
- Purification reservoir to collect diastereomeric salt of the increasingly purified enantiomer
- Filtrate collection reservoir to collect filtrate after each re-crystallization
- Accessories to help separate crystals
Key Benefits:
- Obtain pure enantiomer (up to 90% of theoretical yield) using minimal quantity of racemate
- Up to 90% of the racemate used during screening can be recovered
- Establish a robust procedure for separation through iterative crystallizations needed to achieve target purity
- Produce multi-gram quantities of enantiomer for using in preclinical testing
